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Denaturation of wild-type and mutant HhaI DNA methyltransferases in the presence of urea. M.HhaI (100 nM) was incubated with urea (0.1–4 M) for 5 min at 37°C and the activity of the protein measured in the presence of denaturant. Fractional activity plotted against urea concentration for Trp41 mutants (Ala, Ile and Tyr) and Phe18 mutants (Leu, Cys and Tyr) is shown.
