Figure 4.

Both 5′and 3′ leader sequences are required for inhibition of dimerization. Approximately 3 pmol of transcripts starting at position 1, 126, 185 and 397 and ending at position 444 or 544 were incubated at optimal dimerization conditions as described in the legend to Figure 2. Samples were loaded on a non-denaturing TBE gel (A) and a non-denaturing TBM gel (B). After gel drying, phosphor imager analysis was performed to calculate the dimerization efficiencies that are plotted in bar graphs. Black and white bars represent dimerization of transcripts terminating at position 444 and 544.