Figure 6.

The effect of NC protein on the HIV-2 leader RNA conformation. Approximately 2 pmol of radiolabeled transcript 1/544 was incubated either alone (lanes 1–2) or with a 50-fold molar excess of the antisense DNA oligonucleotide 418/399 (lanes 3–4) at optimal dimerization conditions (see Fig. 2 legend). After slow cooling to room temperature, 1.2 µg synthetic HIV-1 NCp7 protein was added (lanes 2 and 4) and all samples were incubated for 2 h at 37°C, followed by treatment with SDS and a phenol extraction. The positions of dimer (D), monomer (M) and fast-migrating monomer (LDI) conformations are indicated. Lanes 3 and 4 are shown at two intensities.