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Scheme of immobilized molecular beacon array. MB1 and MB2 are two different immobilized molecular beacons. Target T2 is complementary to MB2 and non-complementary to MB1. (A) Before hybridization. (B) After hybridization with T2. MB2 forms a rigid probe–target duplex with T2 and the fluorescein and the quencher are forced to separate, while MB1 maintains the hairpin structure.
