Figure 8.

(A) Sequencing result of the PCR product with an ABI Prism 377. The arrow indicates the C/T heterozygote of apoE codon 158. (B) Electrophoretic image. Lanes 1 and 2 depict the 218 bp PCR product before and after HaeII digestion, respectively. Mutation from C to T causes loss of the restriction site. Lane 2 depicts a C/T heterozygote (15). (C) Electrophoretic image. Lanes 1 and 2 are symmetric and asymmetric PCR products, respectively. Lanes 3 and 4 are PCR products before and after fragmentation with UNG, respectively. (D) and (E) are the fluorescence images before and after hybridization with asymmetric and fragmented PCR products, respectively. The hybridization solutions containing 20 µl PCR products and 80 µl hybridization buffers (20 mM Tris–HCl pH 8.0 and 10 mM MgCl₂). (F) The fluorescence intensity increments after hybridization with asymmetric and fragmented PCR products.