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MD-PASA schemes. (A) An MD-PASA scheme of site couples was made to allow analysis of physical linkage between all nine sites. Order of amplifications would be, in this case, A-G, C-G, C-I, E-I, D-I, D-H, B-H and B-F. (B) A scheme for haplotype analysis of individuals who are heterozygous for sites A to D (like individual 3). (C) A scheme for haplotype analysis of individuals who are heterozygous for sites F to I (like individual 4).
