Figure 2.

Gcs1p and Age2p defects compromise Sec14p pathway function. Isogenic sets of GCS1, AGE2 (WT) and Δgcs1 or Δage2 yeast strains (as indicated) were incubated on YPD agar at 26 (A), 33.5 (B), and 37°C (C) for 72 h. Other relevant genotypes are given for the WT panel, and this genotype organization was maintained in the Δgcs1 and Δage2 panels. (D) Effect of acute Gcs1p dysfunction on sac1-mediated bypass Sec14p. Mutant Δgcs1 strains carrying a sac1 mutation either in combination with a YCp(gcs1-3^ts) plasmid, or not (as indicated), were streaked for isolation on YPD agar plates. Growth was scored after 48 h of incubation at 26 and 37°C. Positive and negative growth controls included a wild-type strain and an isogenic sec14-1^ts strain as indicated. Strains used were as follows: CTY182 (SEC14), CTY1-1A (sec14-1^ts), CTY100 (sec14-1^ts, sac1), and CTY1515 [sec14-1^ts, sac1, Δgcs1, YCp(gcs1-3^ts); Table 1].