Figure 7.

Gcs1p PH domain is dispensable. Gcs1p binds PIPs and harbors a PH domain at its C terminus (Blader et al., 1999; Figure 1B). A gcs1 truncation allele (gcs1-2; Ireland et al., 1994) that deletes the PH domain (codons 227–352; Figure 1B) was expressed from a GCS1 promoter cassette carried on a yeast episomal plasmid. This YEp(gcs1-2) plasmid was transformed into isogenic sec14-1^ts Δgcs1 strains carrying either pct1 or kes1 bypass Sec14p mutations. Transformants were streaked on YPD medium at 37°C and scored after 48 h.