. 2002 Jul;13(7):2193–2206. doi: 10.1091/mbc.01-11-0563.

Table 2.

Gcs1p defects compromise Sec14p pathway function. Isogenic pairs of GCS1 and Δgcs1 yeast strains of the designated genotype were incubated on YPD agar at 26, 33.5, and 37°C for 72 h as indicated. Relative growth properties were then scored. Chronic Gcs1p deficiency strongly exacerbates sec14-1^ts-associated growth defects and cki1-, pct1-, and kes1-mediated bypass Sec14p. These dramatic growth defects are also shown in Figure 2. Results obtained for other mutations (e.g., Δarf1, Δarf2, Δarl1, Δarl2, Δgea1) in this same genetic background are also shown in this Table.

Genotype	26°C	30°C	33.5°C	37°C
SEC14	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
SEC14 Δgcs1	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts Δgcs1	⁺⁺	⁺	−	−
sec14-1^ts Δgcs1/YCp(SEC14)	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts Δarf1	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts Δarf2	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts Δarl1	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts Δarl2	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts Δgea1∷HIS3	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts pct1	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts pct1 Δgcs1	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts pct1 Δgcs1/YCp(SEC14)	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts kes1	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts kes1 Δgcs1	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	−
sec14-1^ts kes1 Δgcs1/YCp(SEC14)	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts kes1 Δgea1∷HIS3	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts cki1	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺
sec14-1^ts cki1 Δgcs1	⁺⁺⁺	⁺⁺⁺	⁺	−
sec14-1^ts sac1	⁺⁺	⁺⁺	⁺⁺	⁺⁺
sec14-1^ts sac1 Δgcs1	⁺⁺	⁺⁺	⁺⁺	⁺