Table 3.

Specificity of ARFGAP involvement in Sec14p pathway function. Isogenic pairs of yeast strains of the designated genotype were incubated on YPD agar at 26, 33.5, and 37°C for 72 h as indicated. Relative growth properties were then scored. Only chronic Age2p deficiency exacerbates sec14-1^ts-associated growth defects and has any obvious effect on bypass Sec14p, specifically sac1-mediated bypass Sec14p.

Genotype	26°C	30°C	33.5°C	37°C
SEC14	+++	+++	+++	+++
SEC14 Δage2	+++	+++	+++	+++
sec14-1ts	+++	+++	+	−
sec14-1ts Δage2	++	++	−	−
sec14-1ts Δage1	+++	+++	+	−
sec14-1ts pct1	+++	+++	+++	+++
sec14-1ts pct1 Δage2	+++	+++	++	+
sec14-1ts pct1 Δage1	+++	+++	+++	+++
sec14-1ts kes1	+++	+++	+++	+++
sec14-1ts kes1 Δage2	+++	+++	+++	+++
sec14-1ts kes1 Δage1	+++	+++	+++	+++
sec14-1ts sac1	++	++	++	++
sec14-1ts sac1 Δage2	++	++	++	−/+
sec14-1ts sac1 Δage1	N/D	N/D	N/D	N/D

N/D, not determined.