Table 1.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| W303 | ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| W303-AR | W303 MATa RDN::ADE2 sir2Δ::TRP1 LEU2::SIR2 | L. Guarente |
| W303-AR sir2-N345A | W303 MATa RDN::ADE2 sir2Δ::TRP1 LEU2::sir2-N345A | L. Guarente |
| W303-AR sir2Δ | W303 MATa RDN::ADE2 sir2Δ::TRP1 | L. Guarente |
| YGM1 | W303 MATα hmr-e (331-324 and 274-256; Rap1 and Abf1 binding sites deleted) | G. Micklem |
| JRY4013 | W303 MATα ADE2 lys2Δ | |
| JRY4563 | JRY4013 sir2Δ::TRP1 | |
| JRY4580 | JRY4013 sir4Δ::TRP1 | |
| JRY4605 | JRY4013 sir3Δ::TRP1 | |
| JRY4623 | JRY4013 sir1Δ::TRP1 | |
| JRY5472 | JRY4013 hmre∗∗ (Rap1 and Abf1 binding sites mutated) | |
| JRY7131 | W303 matΔ::ADE2 FRT-4xGal4-Rap1-Abf1 HMRa ΔI-FRT SIR1 LEU2::FLP1 [cir°] plus pJR1811 | |
| JRY7296 | JRY4013 sir1Δ::LEU2 sir2Δ::TRP1 | |
| JRY7297 | JRY4013 sir1Δ::LEU2 sir3Δ::TRP1 | |
| JRY7298 | JRY4013 sir2Δ::LEU2 sir3Δ::TRP1 | |
| JRY7299 | JRY4013 SIR1-3HA | |
| JRY7300 | JRY4013 SIR1-3HA sir2Δ::TRP1 | |
| JRY7301 | JRY4013 SIR1-3HA sir3Δ::TRP1 | |
| JRY7302 | JRY4013 SIR1-3HA sir4Δ::TRP1 | |
| JRY7303 | W303 MATα 4xGal4-Rap1-Abf1 HMRa ΔI sir2Δ::LEU2 SIR1 plus pJR1811 | |
| JRY7304 | W303 MATα 4xGal4-Rap1-Abf1 HMRa ΔI sir3Δ::LEU2 SIR1 plus pJR1811 | |
| JRY7305 | W303 MATα 4xGal4-Rap1-Abf1 HMRa ΔI sir4Δ::LEU2 SIR1 plus pJR1811 | |
| JRY7306 | W303 MATα 4xGal4-Rap1-Abf1 HMRa ΔI sir1Δ::LEU2 plus pJR1811 and pCF448 |