Figure 6.

Gel-shift supershift and cold-competitor controls for transcription factors dependent on the EGFR (CREB, Egr, Ets, and Stat3) or MAPK (C/EBP). Control nuclear extracts (0) were from untreated cells. Supershift antibodies against CREB (all forms), Stat3, Egr-1, Ets-2, C/EBP-α, and C/EBP-β were added to reactions after nuclear extract incubation with the CREB, Stat3, Egr, Ets, and C/EBP oligonucleotides, respectively (SS). A 20-fold excess of nonradioactive oligonucleotides were added to nuclear extracts as a “cold” competitor (CC).