Reagents and tools table
| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
| Experimental models | ||
| C57BL/6J mice (M. musculus) | Jackson Laboratories | RRID: IMSR_JAX:000664 |
| ASC deficient mice in C57BL/6J background (Pycard–/–) | Mariathasan et al, 2004 | N/A |
| HEK293T cells (H. sapiens) | American Type Culture Collection | CRL-11268 |
| HEK293T cells (H. sapiens) stably expressing human NLRP3 p.D303N | Baroja-Mazo et al, 2014 | N/A |
| Human primary whole blood | This study | N/A |
| Recombinant DNA | ||
| NLRP3 wild-type tagged with Yellow Fluorescent Protein (YFP) | Baroja-Mazo et al, 2014 | N/A |
| ASC tagged with Red Fluorescent Protein (RFP) | Franklin et al, 2014 | N/A |
| ASC tagged with Yellow Fluorescent Protein (YFP) | Baroja-Mazo et al, 2014 | N/A |
| NLRP3 p.Q308H tagged with Yellow Fluorescent Protein (YFP) | This study | N/A |
| Antibodies | ||
| PE anti-ASC (TMS-1) Antibody | Biolegend | Cat. 653902 (clone HASC-71) |
| Vector ImmPress anti-mouse | Vector Laboratories | Cat. MP-7402 |
| FITC Mouse Anti-Human CD14 | BD Biosciences | Cat. 557153 (clone M5E2) |
| APC Mouse Anti-Human CD15 | BD Biosciences | Cat. 551376 (clone HI98) |
| Oligonucleotides and other sequence-based reagents | ||
| pcDNA3.1/V5-His Topo | Life Technologies | Cat. K480001 |
| Chemicals, Enzymes and other reagents | ||
| Mouse IL-1 beta ELISA | Thermo Fisher Scientific | Cat. 88-7013-88; RRID:AB_2574946 |
| Mouse IL-6 ELISA Kit - Quantikine | R&D Systems | Cat. M6000B-1; RRID: AB_2877063 |
| Mouse SAA/SAA1 ELISA Kit | Biorbyt | Cat. ORB170167 |
| MycoProbe Mycoplasma Detection Kit | R&D Systems | Cat. CUL001B |
| ASC oligomers | This study | N/A |
| Penicillin-Streptomycin | Corning | Cat. 30-002-CI |
| Dulbecco’s modified Eagle’s medium (DMEM-F12) w/o L-Glutamine, w/o Hepes Sterile Filtered | Biowest | Cat. L0090-500 |
| RPMI-1640 Medium liquid without L-glutamine | Sigma | Cat. R0883 |
| L-Glutamine | Gibco | Cat. 25030-032 |
| Fetal Bovine Serum (FBS) | Biowest | Cat. S181A |
| OptiMEM reduced Serum Media | Gibco | Cat. 51985-026 |
| GlutaMAX | Thermo Fisher Scientific | Cat. 35050038 |
| Lipofectamine 2000 | Invitrogen | Cat. 11668-019 |
| Vitamin-free casein | Merck Millipore | Cat. C6554 |
| HEPES | Sigma-Aldrich | Cat. H3375 |
| Sucrose | Sigma-Aldrich | Cat. S7903 |
| KCl | Panreac | Cat. 141494 |
| MgCl2 | Sigma-Aldrich | Cat. M2670 |
| EDTA | Sigma-Aldrich | Cat. E5134 |
| EGTA | Sigma-Aldrich | Cat. E4378 |
| CHAPS | Calbiochem | Cat. 220201 |
| Percoll | Merck Millipore | Cat. P4937 |
| Mayer’s haematoxylin | Panreac AppliChem | Cat. 254766.1211 |
| Alcoholic eosin Y | Panreac AppliChem | Cat. 251301.1211 |
| Target Retrieval Solution, High pH | Agilent-Dako | Cat. GV80411-2 |
| DAB+ Substrate Chromogen System | Agilent-Dako | Cat. GV82511-2 |
| Congo Red Stain Kit/Amyloid Stain Kit | Abcam | Cat. ab150663 |
| Software | ||
| GraphPad Prism version 9 | GraphPad Software Inc | www.graphpad.com |
| FlowJo™ v10.8 Software | BD Life Sciences | www.bdbiosciences.com/en-us/products/software/flowjo-v10-software |
| NIS Elements | Nikon | www.microscope.healthcare.nikon.com/products/software/nis-elements |
| Zen Ver. 3.0 Blue Edition | Karl Zeiss | www.zeiss.com/microscopy/en/products/software/zeiss-zen.html |
| Other | ||
| Nikon Eclipse Ti | Nikon | N/A |
| Zeiss Axio Scope A10 | Karl Zeiss | N/A |
| LSRFortessa | BD Biosciences | N/A |
| FACS Canto | BD Biosciences | N/A |
| Synergy Mx Plate Reader | BioTek | N/A |