Fig. 6. Ribosome biogenesis defects caused by Tig2 deletion.

a Left: Immunoblot monitoring accumulation of selected chloroplast proteins in Col-0 and trigger factor mutant lines. Protein samples were collected from young leaves after 21 days of cold-exposure; right panel: quantification of bands. Mean values derive from three biological replicates, error bars denote SD, two-sided Student’s t-test p-values are given, “n.s.” not significant. b Seedlings were grown for 10 days (DAG) on plates with and without lincomycin. c RNA was isolated from young leaves of Col-0 and tig2 lines after 2 weeks of cold exposure or permanent growth at 21 °C. and analyzed by Northern blot (1 µg per lane). Probes against chloroplast 16S and the possible post-maturation fragments of chloroplast 23S (indicated by arrows and depicted in the lower right panel). Quality of RNA and loading controls and are shown in Supplementary Fig. 13.