Table 1.

PCR oligonucleotides

Name	Sequence (5'→3')1
Gene-specific primers
G-UpF-GWL2	TACAAAAAAGCAGGCTccgatcagttgcaacacatc
G-UpR-Gm2	TCAGAGCGCTTTTGAAGCTAATTCGttgttcctgcaccaccatct
G-DnF-Gm2	AGGAACTTCAAGATCCCCAATTCGccggcgagttccacctgaa
G-DnR-GWR2	TACAAGAAAGCTGGGTggttgagcttgctgtcgata
Common primers
Gm-F	CGAATTAGCTTCAAAAGCGCTCTGA
Gm-R	CGAATTGGGGATCTTGAAGTTCCT
GW-attB1	GGGGACAAGTTTGTACAAAAAAGCAGGCT
GW-attB2	GGGGACCACTTTGTACAAGAAAGCTGGGT

¹Sequences in capital letters are common for all genes amplified and overlap with the Gm or attB primer sequences. Lower-case letters indicate gene-specific sequences, here PA1520. PCR oligonucleotide sequences for all 27 genes amplified in this study are given in Table 2 (see Additional file 1).

²G, gene specific sequence, here PA1520.