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. 2005 May 27;4:15. doi: 10.1186/1475-2859-4-15

Table 1.

Strains and Plasmids

Bacterial strain/plasmid Properties Reference
Bacteria
Lactococcus lactis subsp. cremoris NZ3900 Integration of nisRnisK in the chromosome; integration of the lac operon in the in the chromosome and deletion of lacF resulting in a lactose- negative host strain that can be complemented by lacF [25]
Plasmids
pNZ8148 PnisA, CmR; replicon of rolling circle plasmid pSH71, basic NICE vector, derivative of pNZ8048 [12] [26]
pNZ1709 Lysostaphin gene under control of PnisA, CmR; derivative of pNZ8148 This work
pNZ1710 Lysostaphin gene under control of PnisA, lacF; expression vector for food- grade expression, selection on growth with lactose; derivative of pNZ1709 This work [11]