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. 2005 May 26;24(13):2354–2366. doi: 10.1038/sj.emboj.7600702

Figure 1.

Figure 1

Confirmation by streptavidin pull-downs (A–C) and immunoprecipitations (D–G) of proteins identified copurifying with GATA-1. (A) Streptavidin pull-downs of transcription factors. Biotinylated GATA-1 (top panel) is detected by streptavidin–HRP and is absent from the BirA-only transfected cells. (B) Pull-downs of the MeCP1 complex. (C) Pull-downs of ISWI-containing complexes. SB: streptavidin-bound. (D) Immunoprecipitations (IP) using antibodies against GATA-1, TAL-1, FOG-1 (lanes 2, 3 and 4, respectively) and nucleophosmin as negative control (lane 5). (E) IP of the MeCP1 complex by antibodies against GATA-1 and MTA2 (lanes 2 and 3) and nucleophosmin (lane 4). (F) GATA-1 can be specifically immunoprecipitated by an antibody against MTA2. (G) IP of the ACF/WCRF complex by GATA-1 antibodies. Nuclear extract equivalent to 5% used in each pull-down or IP was loaded as control for input material. IP: immunoprecipitating antibody. Arrows show the detecting antibodies.