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. 2005 May 26;24(13):2354–2366. doi: 10.1038/sj.emboj.7600702

Figure 4.

Figure 4

(A–B) FOG-1 bridges GATA-1 and MeCP1. Nuclear extracts from HeLa cells transfected with the FOG-1 and GATA-1 combinations indicated were immunoprecipitated with FOG-1 (A) or MTA2 antibodies (B) and detected with FOG-1 and GATA-1 antibodies. ΔZn-N and ΔZn-C: GATA-1 N- and C-terminal zinc-finger deletion mutants. Arrows: crossreacting IgG. Asterisk (B): GATA-1 signal. (C) Real-time PCR transcription assays in transfected HeLa cells. GATA-1 activates transcription of pG-OVEC, whereas cotransfection of FOG-1 represses to basal levels. (D) Specific recruitment of Mi-2β by cotransfected GATA-1 and FOG-1 by ChIP assays in HeLa cells. Mi-2β recruitment to the repressed gene requires GATA-1 binding to the promoter.