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. 2005 Jun 9;24(13):2379–2390. doi: 10.1038/sj.emboj.7600711

Figure 2.

Figure 2

6-AU-induced transcription inhibition increases histone occupancy within the PMA1 and ASC1 genes. DY103 cells were treated without (−) or with (+) 6-AU. ChIP analysis was carried out using anti-pol II, -H2B, -H3 or -H4, and PCR products of 5′, middle and 3′ region on PMA1(A) or ASC1(B) are shown. TEL and globin are controls. Ratios of ChIP signals normalized to input and the globin control + and −6-AU were determined. Means and standard deviation from three PCR determinations are shown.