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. 2005 Jun 16;24(13):2254–2264. doi: 10.1038/sj.emboj.7600716

Figure 1.

Figure 1

The interacting partners LimF, ChLim, and Rab21. (A) LimF has three LIM domains. ChLim has an N-terminal CH domain, three LIM domains, and four LRDs. Rab21 is a small GTPase; the dominant-negative (T21N) and constitutively activated (Q66L) mutations are indicated. (B) Interactions of LimF with ChLim and Rab21 were studied in a yeast two-hybrid system. All cells were able to grow in the absence of histidine (H), uracil (U), and tryptophan (W). Positive interaction (+) is indicated by growth in the absence of leucine (L) and by enhanced β-galactosidase activity using X-gal as substrate. ChLim is full-length ChLim, CHChLim is the CH domain of ChLim, and LIMChLim is the Lim domain of ChLim. Images of yeast are representative data for full-length ChLim.