Skip to main content
. 2005 Jun 16;24(13):2391–2402. doi: 10.1038/sj.emboj.7600719

Figure 1.

Figure 1

Both the CREB-binding domain of MECT1 and the TAD domain of MAML2 are required for the MECT1-MAML2 fusion to induce foci formation in RK3E cells. (A) RK3E cells were transfected with the indicated expression plasmids, and stained with crystal violet for foci formation at 3 weeks post-transfection. (B) Diagram of the constructs used in focus assays, and the number of foci generated from the transfection of each of these constructs. The number of foci shown was obtained from the 10 cm plates based on the triplicate experiments. M-M2, M2, M, M1, M-M1, and M-VP stand for MECT1-MAML2 fusion, MAML2, MECT1, MAML1, MECT1-MAML1, and MECT1-VP16, respectively. All these genes were cloned into the pFLAG-CMV2 expression vector, and expressed as FLAG-tagged proteins.