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. 2005 Jun 16;24(13):2391–2402. doi: 10.1038/sj.emboj.7600719

Figure 2.

Figure 2

MECT1-MAML2 fusion induces expression of CREB transcriptional target genes. (A) Expression levels of five target genes (both known and previously unknown regulated by cAMP signaling) in MECT1-MAML2-transfected cells, relative to the empty vector-transfected cells, were determined by SYBR green real-time PCR. (B) Induction of two known CREB targets, ATF3 and Nurr1 genes, wasanalyzed by Western blot analyses in human immortalized parotid HSY cells transfected with the indicated expression constructs. Transfected FLAG-tagged proteins except MECT1-VP16 (its band overlaps with IgG light chain, and the expression is shown on the whole-cell lysate blot) were detected after immunoprecipitation (IP) with anti-FLAG antibodies. M2, M, M-M2, M-M1, and M-VP stand for MAML2, MECT1, MECT1-MAML2, MECT1-MAML1, and MECT1-VP16, respectively.