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. 2005 Jun 16;24(13):2391–2402. doi: 10.1038/sj.emboj.7600719

Figure 6.

Figure 6

p300-binding-deficient mutant exhibits a reduction in the ability to activate the CREB pathway and to induce colony formation in RK3E. (A) The MECT1-MAML2 mutant, Δ48–222, is unable to immunoprecipitate with p300. 293T cells were transfected with constructs expressing HA-tagged p300 and FLAG-tagged full-length or truncation mutants of MECT1-MAML2. The anti-HA immunoprecipitates were immunoblotted with anti-FLAG antibodies. (B) The MECT1-MAML2 (Δ48–222) mutant is not acetylated by p300. 293T cells were transfected with constructs expressing HA-tagged p300 and FLAG-tagged full-length or truncation mutants of MECT1-MAML2. The anti-FLAG immunoprecipitates were immunoblotted with anti-acetylated lysine and anti-FLAG antibodies. (C) The p300-binding-deficient mutant, MECT1-MAML2 (Δ48–222), induced less Nurr1 and ATF3 expression, as compared to the full-length MECT1-MAML2. (D) The p300-binding-deficient MECT1-MAML2 mutant generated a reduced number of colonies in RK3E colony formation assays. The data were based on three independent experiments.