Figure 6.

(A and B) Deletion of the F-actin–binding site of Mena and VASP increases Listeria motility. MV^D7 cells expressing high levels of GFP-Mena ΔFAB (A) or GFP-VASP ΔFAB (B) were infected with wild-type Listeria, fixed, and stained with fluorescent phalloidin. In both cases, Listeria induced the formation of actin tails (arrows in A and B) that were not distinguishable from those induced by the same bacterium in MV^D7 cells expressing wild-type Ena/VASP proteins (compare to Figure 2). Bar, 2 μm. (C). Box and whiskers plots of bacterial speed.