Figure 6.

Effect of rab11BP(334–504) on the recycling of the TR and vpTR. Cells were incubated for 3.5 h with Cy3-Tf, washed, and incubated for an additional 60 min (for the TR) or 120 min (for vpTR) in medium without Cy3-Tf. At the end of the incubation the cells were fixed and the amount of Cy3-Tf remaining was determined. The different efflux times were chosen because the vpTR is recycled more slowly than the TR (Subtil et al., 2000). Data are from a representative experiment (means ± SEM).