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. 2002 Jul;13(7):2502–2517. doi: 10.1091/mbc.01-11-0529

Figure 1.

Figure 1

Caveolin-1-overexpressing MEFs show premature irreversible growth arrest and senescence-like cell morphology. (A) MEFs derived from normal control mice and from caveolin-1 transgenic mice were passaged into a new 10-cm dish (1:3 division) every time they reached subconfluency. The number of passages MEFs were passaged before showing irreversible growth arrest was recorded. Note that overexpression of caveolin-1 induces premature growth arrest in MEFs. Values represent means ± SEM *P < 0.001. (B) Cells representative of the two MEF cell populations are shown (×60 magnification). (C) MEFs derived from normal control mice and from caveolin-1 transgenic mice were examined using a BX50WI Optical light microscope (Olympus) at a magnification of ×10. The percentage of cells showing senescence-like cell morphology was recorded. Note that 70 ± 5% of MEFs (passage 1) overexpressing caveolin-1 shows a large and flat morphology as compared with 15 ± 3% of MEFs derived from normal control mice. Values represent means ± SEM. *P < 0.0005.