Figure 12.
Secretion of glycoproteins by emp46/47 deletion strains. Cells were labeled with [35S]methionine for 10 min and were chased for 30 min. Media were collected and incubated with ConA-Sepharose to isolate extracellular glycoproteins. Proteins were separated by SDS-PAGE, and labeled proteins were detected by autoradiography. ● in A and ○ in B mark glycoproteins secreted inefficiently by mutant cells. Arrowheads designate glycoproteins secreted underglycosylated by the emp47Δ cells. (B) Low molecular weight region with increased samples.