Skip to main content
. 2002 Jul;13(7):2518–2532. doi: 10.1091/mbc.E02-01-0027

Figure 6.

Figure 6

(A) The C-terminal region of Emp46p and other traffic lectins. ER-targeting dilysine signals are boldfaced, and the tyrosine-containing motifs are underlined. Numbering is from the carboxy-terminal end with the last amino acid indicated as −1. (B) Influence of the C-terminal region of Emp46p on its intracellular steady-state localization. Wild-type GFP-Emp46p (a), a series of point mutants in the tyrosine-containing motif (b-d), or a mutated dilysine motif (e and f) was expressed in KSY007 (emp46Δ) containing pRS412(ADE2). Each construct is denoted by mutated tyrosine-containing motif (YYMF at positions 13–16 from the C-terminal end) or last five amino acids (KVKLL at positions 1–5 from the C-terminal end). The left panels show the GFP patterns, and the right panels show the corresponding Nomarski images. Bar, 5 μm.