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. 2002 Jan 8;99(2):585–590. doi: 10.1073/pnas.012611299

Figure 1.

Figure 1

(A) The IleRS-catalyzed editing reaction in the presence of tRNAIle and valine. (B) Closeup view of the posttransfer editing site. D279 of T. thermophilus ValRS (which aligns with D342 of E. coli IleRS) is positioned to interact with a misactivated amino acid that would be attached to either the 2′-OH or the 3′-OH of A76 in a posttransfer complex. The D279 side chain is too far away to directly interact with the ribose hydroxyls or to interact with the misactivated aminoacyl-AMP in a possible pretransfer complex. Coordinates of the ValRS/tRNAVal complex were taken from Fukai et al. (19). ValRS and tRNAVal are colored by atom type (green, carbon; red, oxygen; blue, nitrogen). Hydrogen atoms and protein backbone oxygen atoms are not shown.