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. 2002 Jan 8;99(2):585–590. doi: 10.1073/pnas.012611299

Figure 5.

Figure 5

Kinetic analysis of D342E IleRS editing. ATP hydrolysis editing reactions (as in Fig. 2A) were carried out at various concentrations of tRNAIle. The reciprocals of the cpm of hydrolyzed ATP were plotted against the reciprocal of tRNAIle concentration. KM app for tRNAIle was defined as the negative reciprocal of the x-intercept of the resulting lines. Inset shows the equivalent experiment for the IleRS catalyzed aminoacylation reaction (formation of Ile-tRNAIle) by using various concentrations of tRNAIle.