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Loss of PYR complex DNA binding activity. Gel shift assay of E14.5 fetal liver nuclear extract using labeled δ60ym and YY1 as probes. (Left) DNA binding activity of PYR complex is present in the wild-type mice (+/+), reduced in the heterozygotes (+/−), and completely absent in the null mice (−/−). (Right) Binding of YY1 protein to its binding site shows the quality of the protein extracts in all of the samples.
