Skip to main content
. 2002 Jan 22;99(2):602–607. doi: 10.1073/pnas.022412699

Figure 4.

Figure 4

Delayed mouse globin switching. Primer extension assay of fetal liver globin RNA and globin chain electrophoresis of yolk sac and fetal blood from E14.5 mice. (A Upper) Primer extension results using primers for mouse embryonic β-globin (Ey-globin and βh1) and adult β-globin. (A Lower) Embryonic globin to (embryonic + adult) globin ratios from primer extension data quantified with a phosphorimager. Ikaros null mice have relatively higher persistence of mouse embryonic globin RNA. (B Upper) Electrophoresis of globin chains using blood from E14.5 mice. (B Lower) Embryonic globin to (embryonic + adult) globin ratios from TAU gel data quantified with a densitometer. Null mice have higher levels of both embryonic α-globin and embryonic β-globin in their blood at E14.5.