Table 2.
Differences in expression levels of hematopoietic transcripts in E14.5 liver between Ikaros −/− and Ikaros +/+ embryos
| Class | Down-regulated genes | Unchanged genes | Up-regulated genes |
|---|---|---|---|
| Red cell membrane proteins | Aquaporin 1 (0.3)* | Erythroid alpha-spectrin† | |
| Transferrin receptor† (0.4) | Erythroid ankyrin 1 | ||
| Band 4.2† (0.6) | Glycophorin A | ||
| Rh30 (0.8) | Band 3 | ||
| Erythropoietin receptor (0.8) | Band 4.1 | ||
| Red cell enzymes | Red cell acid phosphatase (0.4) | ||
| Erythroid ALA synthase (0.7) | |||
| Erythroid nuclear proteins | EKLF† (0.6) | Friend of GATA-1 (FOG) GATA-1† | NF-E2, 45-kDa subunit (1.4) |
| Hemoglobins | Adult α-globin chain | Embryonic × globin (α-like), chain (1.3) | |
| Adult β major globin chain | Embryonic βh0 and βh1 globin chains (1.6) | ||
| Embryonic y-globin (β-like) chain | |||
| Hematopoietic | Wnt10b (<0.1) | TAL1 | MLL (1.6) |
| Ikaros† (0.1) | PU.1† (3) |
*
Relative null/wild-type expression ratio. This ratio is the average of E14.5 liver expression levels from five Ikaros −/− (null) and five Ikaros +/+ (wild-type) embryos. Expression ratios are shown only for transcripts that have statistically significant differences in expression (P < 0.05, t test). Unchanged genes have no statistically significant difference in expression between wild-type and Ikaros null embryos.
†
Results confirmed by real time reverse-transcriptase-PCR analysis.