Fig. 3. Recombination intermediates potentiate mitotic death.
a, Western blots of whole-cell extracts from 3F HeLa (representative of n = 2). b,c, First mitosis outcome (b) and mitotic duration (c) in G2-enriched 3F HeLa treated with 14 Gy IR (for b, the mean n = 2, two-sided Fisher’s exact test of N; for c, mean ± s.e.m. and two-tailed Kolmogorov–Smirnov test of N). d, Western blots of whole-cell extracts collected 1 h post IR from HeLa ± 0.2 µM VE-822 (n = 1). e, RAD51 foci in HeLa ± siRNA (si) and/or 0.2 µM VE-822 (mean ± s.e.m. of n = 5, 4, 4, 3, 5, 5, 5 and 3 from left to right; violin plots of N, the dotted lines are quartiles and the solid lines medians; ordinary one-way ANOVA with Fisher’s LSD multiple comparisons test). All VE-822 negative conditions include replicates from Fig. 2i. f,g, First mitosis (f) and all cell cycle outcomes over 120 h (g) in 3F HeLa ± siRNA, 0.2 µM VE-822, 0.5 µM reversine and/or 14 Gy IR (for f, mean n = 2, two-sided Fisher’s exact test of N; for g, the mean ± s.e.m. n = 2, two-sided Fisher’s exact test of N for mitotic death). h, Cell death from g (mean ± s.e.m. n = 2, two-sided Fisher’s exact test of N). i, First mitosis in G2-enriched 3F HeLa following 2 Gy IR (mean n = 2, except RTEL1 siRNA ± BRCA2/PALB2 siRNA mean ± s.e.m. (n = 3); two-sided Fisher’s exact test of N). For a–i, N = individual cells across all replicates and n = biological replicates. n.s., not significant.