Fig. 4. Chromosomal structural aberrations correlate with first mitosis survival.
a, Cytogenetic preparations from HeLa ± 14 Gy. Scale bar, 10 µm (representative of n = 3). b–e, Radial chromosomes (b), chromatid-type fusions (c), multi-centric chromosomes (d) and ring chromosomes (e) from asynchronous mock irradiated or the first mitosis post 14 Gy IR in G1, S or G2-enriched HeLa (mean ± s.e.m. n = 3, N = 50 metaphases per replicate, ordinary one-way ANOVA with Fisher’s LSD multiple comparisons test). f,g, Radial chromosomes (f) and chromatid-type fusions (g) in asynchronous HeLa following 14 Gy IR (mean ± s.e.m. n = 4, 4, 2, 2 and 4 from left to right, N = 50 metaphases per replicate; two-sided Fisher’s exact test of N). h,i, HeLa radial chromosomes (h) and chromatid-type fusions (i) in the first mitosis post 14 Gy IR (mean n = 2, except 0 Gy control siRNA (n = 4), N = 50 metaphases per replicate, two-sided Fisher’s exact test of N). The control siRNA are the 0 and 36 h timepoints from f and g.