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Confirmation of the conjugated diene compounds by formation of MTAD adducts. (A and B) Control Sf21 cells with addition of E9-14:Acid produced a diene with retention time (A) and diagnostic ions (B) for Z9,E11-16. (C and D) Sf21 cells infected with bacmid viruses, which were transposed with pFastBac1-LBAM-PGE11-ORF, produced an additional diene with retention time (C) and diagnostic ions (D) for E9,E11-14:Acid.
