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Effect of pyruvate on the release of FFA in vitro from adipose tissue. Two pieces (30–100 mg) from the distal thin part of epididymal fat pads from PPARE+/− (+/−; three mice) and PPARE−/− (−/−; four mice) mice after an 18-h fasting were incubated in vitro with (dotted bars) or without (hatched bars) 25 mM pyruvate. FFA levels (per gram of adipose tissue) in the incubation medium were measured after a 3-h incubation at 37°C.
