Table 2.
Biochemical analysis of PPARE−/− and littermate control mice
| Metabolites | Genotype
|
|||
|---|---|---|---|---|
| +/+ | +/− | −/− | −/−* | |
| βHB | ||||
| Fed (mM) | 0.4 ± 0.07 (4) | 0.14 ± 0.03 (7) | 0.18 ± 0.03 (16) | 0.09 ± 0.04 (3) |
| Fasted (mM) | 1.2 ± 0.23 (4) | 1.1 ± 0.1 (7) | 1.06 ± 0.13 (16) | 0.36 ± 0.2 (3) |
| TG | ||||
| Fed (mg/dl) | 106 ± 16 (4) | 90.8 ± 10 (8) | 84.8 ± 4 (18) | 73 ± 8 (3) |
| Fasted (mg/dl) | 98 ± 15 (4) | 89 ± 10 (9) | 66.8 ± 4.6 (18) | 80 (2) |
| Insulin fed (ng/ml) | 0.96 ± 0.22 (4) | 1.37 ± 0.71 (3) | 1.16 ± 0.29 (9) | 1.5 ± 0.4 (3) |
| Glucose fasted (mg/dl) | 89 ± 10 (4) | 101 ± 8 (11) | 93 ± 6 (18) | 137 ± 20 (4) |
| FFA | ||||
| Fed (mM) | ND | 0.67 ± 0.3 (6) | 0.86 ± 0.2 (10) | 0.87 ± 0.3 (3) |
| Fasted (mM) | ND | 0.7 ± 0.3 (6) | 0.7 ± 0.1 (10) | 0.56 ± 0.1 (3) |
Serum and whole blood analysis as described in Methods. Genotype: PPARE+/+ (+/+), PPARE+/− (+/−), PPARE−/− (−/−), and PPARE−/− with lipodystrophy (−/−
*
). ± indicates the SEM calculated. In parentheses is the number of mice used. βHB, β-hydroxybutyrate; TG, triglycerides; ND, not determined.