Table 2.
Oligonucleotides
| Real time quantitative PCR probes | |||
|---|---|---|---|
| DUX4L |
5’-TCAGCCAGAATTTCACGGAAG-3’ 5’-CCATTCTTTCCTGGGCATCC-3’ |
FAM/ZEN/3IABκFQ | IDT |
| ZSCAN4 |
5’-CTAGTCACACATCAGCTCAGT-3’ 5’-TTCAGTCTCTTGCCTTGTGTC-3’ |
FAM/ZEN/3IABκFQ | IDT |
| TRIM49B |
5’-GCTGCTGAGGAACACCA-3’ 5’-ATTGCTTCTAGCCTTAAATTCACAT-3’ |
FAM/ZEN/3IABκFQ | IDT |
| TRIM48 |
5’-ATCACTGGACTGAGGGACA-3’ 5’-GGGCGGATTTTGACGGT-3’ |
FAM/ZEN/3IABκFQ | IDT |
| ICP0 |
5’-CGGACACGGAACTGTTCGA-3’ 5’-CGCCCCCGCAACTGC-3’ |
FAM/ZEN/3IABκFQ | IDT |
| HPRT1 |
5’-CGAGATGTCATGAAGGAGATGG-3’ 5’-TCAGCAAAGAACTTATAGCCCC-3’ |
HEX/ZEN/3IABκFQ | IDT |
| VTRNA1 | 5’-TTTAATTGAAACAAGCAACCTGTCT-3’ | FAM/NFQ | Thermo Fisher Scientific |
| TRIM43 |
5‘-TCATCTGATTCGACGGCAAG-3´ 5´-AGATGGTGACAGGGTCTACC-3´ |
FAM/ZEN/3IABκFQ | IDT |
| ICP27 |
5’-ACATCTTGCACCACGCCAG-3’ 5’-CGCCAAGAAAATTTCATCGAG-3’ |
FAM/ZEN/3IABκFQ | IDT |
| gG |
5’-GCAGGCAYACGTAACGCACGCT-3’ 5’-TTCTCGTTCCTCACTGCCTCCC-3’ |
5Cy5/ 3IAbRQSp | IDT |
| BZLF1 |
5’-TTCAGAATCGCATTCCTCCAG-3’ 5’-CTAGCAGACATTGGTGTTCCA-3' |
FAM/ZEN/3IABκFQ | IDT |
| BRLF1 |
5’-CAGGAGTTAGCCTCAGAAAGTC-3’ 5’-ATACCATACAGGACACAACACC-3' |
FAM/ZEN/3IABκFQ | IDT |
| EBNA1 |
5’-CACATGTCGTCTTACACCATTGA-3’ 5’-TGTGGGCCGGGTCCA-3' |
FAM/ZEN/3IABκFQ | IDT |
| PCR primer for EMSA assay | |||
| HSV1-BS1 |
fwd: 5’-/5Cy5/ GTGTACCACTGCCACTGTCG-3’ rev: 5’-/5Cy5/ GTCTGATCATGCCCCATACC-3’ |
IDT | |
| HSV1-BS2 |
fwd: 5’-/5Cy5/ ACATGACGTTCCACAGGTCC-3’ rev: 5’-/5Cy5/ TCACCGAGCCAGAAACTACG-3’ |
IDT | |
| HSV1-No-BS |
fwd: 5’-/5Cy5/ CGTGAACCAAAGACGAGGGC-3’ rev: 5’-/5Cy5/ CCACGTTGAGAAGCTCGTCG-3’ |
IDT | |
| KSHV-BS1 |
fwd: 5’-/5Cy5/ ACGTACTAAATCTACTTGACAC-3’ rev: 5’-/5Cy5/CAGACACTATCCCAGAAAC-3’ |
IDT | |
| KSHV-BS2 |
fwd: 5’-/5Cy5/CTCTGTAGAGGTGATGTCC -3’ rev: 5’-/5Cy5/ATTATTATGAGGCCTATCTG -3' |
IDT | |
| KSHV-BS3 |
fwd: 5’-/5Cy5/ATCTACATATAGCGTCTACACC-3’ rev: 5’-/5Cy5/AGGTTACATTAGTAGTGTCACG-3' |
IDT | |
| PCR primer for HSV-1 Standard | |||
| HSV-1 gG |
fwd: 5’-TTGGCACAAAAAGACCCCGA-3’ rev: 5’- AACTAGATACCACCGCCTTTATTG-3’ |
IDT | |
| PCR primer for nanobody cloning | |||
| Nanobody |
fwd: 5’- AGAGCTCGTTTAGTGAACCGTC-3’ rev: 5’- GGTGGACCGGTTCATTACTAAAC-3’ |
IDT | |
| Backbone |
fwd: 5’- GTTTAGTAATGAACCGGTCCACC-3’ rev: 5’- GGCGATCTGACGGTTCACTAAAC-3’ |
IDT | |