Figure 3.
Effect of trans complementation of TLS RNA (A) and wheat germ tRNAs (B) on the assembly of truncated BMV/ΔTLS RNAs. The indicated 32P-labeled in vitro transcribed RNAs were separated by agarose gel electrophoresis. RNAs run in lanes marked T show the transcripts of genomic RNAs used in assembly assays. RNAs run in lanes marked V show the genomic RNAs recovered from virions assembled in the presence of the trans-complementing B3 TLS RNA (A) or wheat germ tRNA (B). (A) The migration position of the 3′ 201-nt TLS RNA is indicated by an arrow. (B) The position of 32P-end-labeled wheat germ tRNA is indicated by an arrowhead. wt transcripts were loaded as size markers.