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Identification of Ku86 null cell lines by using genomic PCR. (A) A cartoon of the primers used and the expected sizes of the PCR products. (B) Ethidium bromide-stained agarose gel of the resultant PCR products. Genomic DNA was isolated from wild-type (+/+), heterozygous (+/−), Cre-treated heterozygous (+/− Cre), a random colony following the second round of targeting (+/− colony DNA), and a slow-growing colony on the same plate (−/− colony DNA). M, DNA marker.
