Table 2.
Partial list of genes induced or repressed in w1118 flies by 10 days of feeding with PBA at 29°C
Function | Clone ID | Gene | Fold change |
---|---|---|---|
Genes induced in PBA-treated flies (confirmed by Northern blot) | |||
Detoxification | CG8905 | Superoxide dismutase | 51.9 |
CG3656 | Cytochrome P450-4d1 | 7.2 | |
CG10045 | Glutathione S-transferase | 4.6 | |
Chaperon | CG4264 | hsc70 | 4.5 |
CG12101 | hsp60 | 6.7 | |
CG3061 | dnaJ like2 | 2.9 | |
Translation | CG8280 | Translational elongation factor1 α | 4.1 |
Neurotransmitter | CG15444 | inebriated | 26.8 |
Transcription factor | CG5102 | daughterless | 8.0 |
Ligand binding | CG7398 | transportin | 8.0 |
Signal transduction | CG7291 | Epididymal secretory protein | 7.5 |
Transporter | CG4994 | Mitochondrial phophate carrier protein | 5.1 |
Growth factor | CG4472 | Imaginal disc growth factor1 | 5.3 |
Genes repressed in PBA-treated flies (confirmed by Northern blot) | |||
Metabolism | CG12055 | Glyceraldehyde-3-phosphate dehydrogenase1 | 3.7 |
CG2286 | NADH:ubiquinone reductase 75-kD subunit precursor | 25.3 | |
FBgn0013674 | Cytochrome c oxidase | 6.6 | |
CG3832 | Peptidyl gycine α hydroxylating monooxygenase | 1.4 | |
CG3523 | Fatty acid synthetase | 2.4 | |
CG14235 | Cytochrome c oxidase subunitVIb | 2.2 | |
CG3001 | Hexokinase | 5.0 | |
Chaperon | CG10578 | dnaJ like1 | 13.2 |
DNA binding | GH03026 | osa | 5.0 |
Ligand binding | GH25160 | calreticulin | 26.5 |
Signal transduction | GH03076 | Peroxisomal farnesylated protein | 1.8 |
Kinase | GH21935 | Cyclin-dependent kinase9 | 2.7 |
Ion channel | GH08586 | porin | 1.4 |
All were confirmed by reverse-Northern blots and fold change is indicated for each. The first seven of the induced genes were chosen for confirmation because of previous literature indicating their involvement in aging; six others were chosen at random from the remaining set of induced clones. The 13 genes in the repressed group were chosen at random. Data are based on two separately prepared batches of mRNA, using the same membrane before and after stripping.