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. 2002 Jan 15;99(2):838–843. doi: 10.1073/pnas.022631999

Table 2.

Partial list of genes induced or repressed in w1118 flies by 10 days of feeding with PBA at 29°C

Function Clone ID Gene Fold change
Genes induced in PBA-treated flies (confirmed by Northern blot)
Detoxification CG8905 Superoxide dismutase 51.9
CG3656 Cytochrome P450-4d1 7.2
CG10045 Glutathione S-transferase 4.6
Chaperon CG4264 hsc70 4.5
CG12101 hsp60 6.7
CG3061 dnaJ like2 2.9
Translation CG8280 Translational elongation factor1 α 4.1
Neurotransmitter CG15444 inebriated 26.8
Transcription factor CG5102 daughterless 8.0
Ligand binding CG7398 transportin 8.0
Signal transduction CG7291 Epididymal secretory protein 7.5
Transporter CG4994 Mitochondrial phophate carrier protein 5.1
Growth factor CG4472 Imaginal disc growth factor1 5.3
Genes repressed in PBA-treated flies (confirmed by Northern blot)
Metabolism CG12055 Glyceraldehyde-3-phosphate dehydrogenase1 3.7
CG2286 NADH:ubiquinone reductase 75-kD subunit precursor 25.3
FBgn0013674 Cytochrome c oxidase 6.6
CG3832 Peptidyl gycine α hydroxylating monooxygenase 1.4
CG3523 Fatty acid synthetase 2.4
CG14235 Cytochrome c oxidase subunitVIb 2.2
CG3001 Hexokinase 5.0
Chaperon CG10578 dnaJ like1 13.2
DNA binding GH03026 osa 5.0
Ligand binding GH25160 calreticulin 26.5
Signal transduction GH03076 Peroxisomal farnesylated protein 1.8
Kinase GH21935 Cyclin-dependent kinase9 2.7
Ion channel GH08586 porin 1.4

All were confirmed by reverse-Northern blots and fold change is indicated for each. The first seven of the induced genes were chosen for confirmation because of previous literature indicating their involvement in aging; six others were chosen at random from the remaining set of induced clones. The 13 genes in the repressed group were chosen at random. Data are based on two separately prepared batches of mRNA, using the same membrane before and after stripping. 

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