Table 2.

Partial list of genes induced or repressed in w¹¹¹⁸ flies by 10 days of feeding with PBA at 29°C

Function	Clone ID	Gene	Fold change
Genes induced in PBA-treated flies (confirmed by Northern blot)
Detoxification	CG8905	Superoxide dismutase	51.9
	CG3656	Cytochrome P450-4d1	7.2
	CG10045	Glutathione S-transferase	4.6
Chaperon	CG4264	hsc70	4.5
	CG12101	hsp60	6.7
	CG3061	dnaJ like2	2.9
Translation	CG8280	Translational elongation factor1 α	4.1
Neurotransmitter	CG15444	inebriated	26.8
Transcription factor	CG5102	daughterless	8.0
Ligand binding	CG7398	transportin	8.0
Signal transduction	CG7291	Epididymal secretory protein	7.5
Transporter	CG4994	Mitochondrial phophate carrier protein	5.1
Growth factor	CG4472	Imaginal disc growth factor1	5.3
Genes repressed in PBA-treated flies (confirmed by Northern blot)
Metabolism	CG12055	Glyceraldehyde-3-phosphate dehydrogenase1	3.7
	CG2286	NADH:ubiquinone reductase 75-kD subunit precursor	25.3
	FBgn0013674	Cytochrome c oxidase	6.6
	CG3832	Peptidyl gycine α hydroxylating monooxygenase	1.4
	CG3523	Fatty acid synthetase	2.4
	CG14235	Cytochrome c oxidase subunitVIb	2.2
	CG3001	Hexokinase	5.0
Chaperon	CG10578	dnaJ like1	13.2
DNA binding	GH03026	osa	5.0
Ligand binding	GH25160	calreticulin	26.5
Signal transduction	GH03076	Peroxisomal farnesylated protein	1.8
Kinase	GH21935	Cyclin-dependent kinase9	2.7
Ion channel	GH08586	porin	1.4

All were confirmed by reverse-Northern blots and fold change is indicated for each. The first seven of the induced genes were chosen for confirmation because of previous literature indicating their involvement in aging; six others were chosen at random from the remaining set of induced clones. The 13 genes in the repressed group were chosen at random. Data are based on two separately prepared batches of mRNA, using the same membrane before and after stripping.