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FACS analysis and SLP-76 expression in spleen cells. (a) Surface expression of CD3 and of CD4 and CD8 on spleen cells. Results are representative of three experiments. (b) Expression of SLP-76 protein in purified T cells by intracellular staining with FITC-conjugated rabbit anti-SLP-76 antibody (c) Expression of SLP-76 protein in 1 ×106 splenocytes assessed by Western blotting.
