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Anti CD3 induced proliferation of splenic T cells. Proliferation of purified T cells to anti-CD3 (coated at 5 μg/ml) in the presence or absence of anti-CD28 (coated at 5 μg/ml) and mouse rIL-2 (20 ng/ml), and to PMA (50 ng/ml) + ionomycin (500 μM). Results are representative of three experiments.
