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. 2002 Jan 22;99(2):925–930. doi: 10.1073/pnas.012329699

Figure 3.

Figure 3

Effect of stable transfection of control lymphoblastoid cells (C3ABR) with full-length and mutant forms of ATM on ATM kinase activity and radiosensitivity. (a) C3ABR cells were stably transfected with wild type, SRI 2546del3, or V2716A. ATM protein was induced, and cells were irradiated (IR) as described in the Fig. 2a legend. (Lower) ATM kinase in vivo; (Upper) In vitro ATM kinase using p531–40 as substrate; (Middle) expression of FLAG-tagged ATM determined by immunoprecipitation (IP) with anti-FLAG antibodies followed by immunoblotting with anti-ATM antibodies. WB, Western blot. (b) C3ABR cells stably transfected with R2849P, G2867R, or A2274T and ATM kinase activity and protein expression were determined as described in Fig. 2a the legend. The panels are as described for a. (c) C3ABR cells stably transfected with G2287A, C2464R, or S2592C. ATM protein and kinase were induced and assayed as described for a. (d) C3ABR cells stably transfected with wild-type, V2662 del, or G2772R and ATM kinase activity and expression were determined as described for a.