Fig. 3.

Time course of prostate regression and regeneration reveals androgen-dependent plasticity. A, B Meta-analyses of single-cell RNA-seq datasets for prostate regression and regeneration. A As described in [6], for regression time points, wild-type mice were castrated and prostate tissues from 2 biological replicates were collected at 1 day, 7 days, 14 days, and 28 days after castration; for regeneration time points, mice that had been castrated for at least 4 weeks were subcutaneously implanted with dihydrotestosterone (DHT) pellets, with 2 biological replicates collected at 1, 2, 3, 7, 14, and 28 days after pellet implantation. B As described in [5], prostate tissues were collected from wild-type mice at 7 and 28 days after castration. “Cell type score” is defined as the percentage of most specific differentially expressed genes for each population, averaged over the whole population (“Methods”). Changes in gene expression that are enriched in urethral but not PrU cells, such as Areg and Ociad2, in the LumA (C), basal (D), and LumP (E) populations, showing distinguishing genes for each population (left column), genes for general compartmental markers, and genes that are enriched for PrU and not co-expressed in LumP (right column), where the line indicates the average expression for each gene across the population and the bar indicates confidence interval (± 95%)