Fig. 4.

Reference plots for human prostate scRNA-seq data. A Aggregated composite UMAP plot for samples of benign human prostate and adjacent benign prostate. B–E Plots of individual datasets. B UMAP plot corresponding to primarily LumAcinar cells taken from the peripheral zone of 1 patient [4]. C Plot containing primarily basal and PrU cells from 1 patient with PCa [5]. D Dataset containing primarily basal, PrU, and LumDuctal cells from 1 patient without PCa [11]. E Dataset containing mixture of prostate and seminal vesicle, originating from 2 organ donor patients with no history of prostate disease [13]. F Dot plot of select top differentially expressed genes (among genes that are expressed in more than 60% of the population and have the highest mean expression in that population) for the epithelial and stromal cell populations from the reference aggregated normal human prostate. The lung club cell marker SCGB1A1 and hillock cell marker KRT13 are highlighted, indicating that these do not clearly correspond to single, distinct prostate cell types. G Heatmap comparing the total gene expression profiles of the cell types in the normal human prostate dataset [13] to those of the aggregated normal mouse prostate, using Wasserstein distance as a metric. Darker color indicates greater transcriptomic similarity. Tables listing the most similar mouse and human epithelial populations based on gene expression, generated by overlaying the mouse cell type signatures onto the human populations (H) and vice versa (I)