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[Preprint]. 2025 Jan 9:2025.01.08.25320225. [Version 1] doi: 10.1101/2025.01.08.25320225

Soluble Immune Checkpoint Protein and Lipid Network Associations with All-Cause Mortality Risk: Trans-Omics for Precision Medicine (TOPMed) Program

Annabelle Rodriguez, Chaojie Yang, Wenqi Gan, Keaton Karlinsey, Beiyan Zhou, Stephen S Rich, Kent D Taylor, Xiuqing Guo, Jerome I Rotter, W Craig Johnson, Elaine Cornell, Russell P Tracy, J Peter Durda, Robert E Gerszten, Clary B Clish, Thomas Blackwell, George J Papanicolaou, Honghuang Lin, Laura M Raffield, Jose D Vargas, Ramachandran Vasan, Ani Manichaikul
PMCID: PMC11741490  PMID: 39830278

Abstract

Adverse cardiovascular events are emerging with the use of immune checkpoint therapies in oncology. Using datasets in the Trans-Omics for Precision Medicine program (Multi-Ethnic Study of Atherosclerosis, Jackson Heart Study [JHS], and Framingham Heart Study), we examined the association of immune checkpoint plasma proteins with each other, their associated protein network with high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), and the association of HDL-C- and LDL-C-associated protein networks with all-cause mortality risk. Plasma levels of LAG3 and HAVCR2 showed statistically significant associations with mortality risk. Colocalization analysis using genome wide-association studies of HDL-C or LDL-C and protein quantitative trait loci from JHS and the Atherosclerosis Risk in Communities identified TFF3 rs60467699 and CD36 rs3211938 variants as significantly colocalized with HDL-C; in contrast, none colocalized with LDL-C. The measurement of plasma LAG3, HAVCR2, and associated proteins plus targeted genotyping may identify patients at increased mortality risk.

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