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. 2025 Jan 17;13:RP95273. doi: 10.7554/eLife.95273

Table 2. Specifications of morphological staining composition.

Staining solution 1
Dye Target Excitation (nm) Emission (nm) Stock concentration End dilution Supplier Catalog #
DAPI Nucleus 350 470 2.5 mg/ml 1/500 Sigma Aldrich D9542
Concanavalin ER 480 490–540 5 mg/ml 1/300 Life Technologies C11252
WGA Golgi 550 560–570 1 mg/ml 1/600 Life Technologies W32464
Phalloidin Actin 570 580–610 66 µM 1/1000 Life Technologies A12380
Mitotracker Mitochondria 640 650–660 1 mM 1/1000 Life Technologies M22426
Staining solution 2
Syto14 Nucleoli/RNA 525 540–580 5 mM 1/1000 Life Technologies S7576