Figure 6.

MW7 scFv inhibits aggregation of mutant htt exon 1. (A) Lysates of 293 cells transfected with mutant htt and an scFv were subjected to high-speed centrifugation and were analyzed by Western blotting. The htt in the pellet that can be solubilized by SDS treatment is ≈80 kDa, whereas the htt that cannot be solubilized does not enter the gel and is visualized as a band at the top of the gel. Very little of either form of htt is found in lysates from cells transfected with MW7 scFv. In contrast, these forms of htt appear to be greater in lysates from cells transfected with MW1 or -2 scFvs than in lysates from control cells. (B) The level of soluble htt in the cleared lysates does not appear to be affected by scFv expression.